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Image Search Results
Journal: Biomedicines
Article Title: Impaired Heat Shock Protein Expression in Activated T Cells in B-Cell Lymphoma
doi: 10.3390/biomedicines10112747
Figure Lengend Snippet: HSP, PD-1, CTLA-4, and STAT3 expression in activated T cells. ( A ) Intracellular expression of HSPs, PD-1, and CTLA-4 (as mean fluorescence intensity, MFI) in activated T cells in healthy controls. Pan T cells were isolated from healthy PBMCs and stimulated with anti-CD3/CD28 or unstimulated (resting) for 48 h. ( B ) Intracellular HSPs, PD-1, and CTLA-4 in indolent and aggressive lymphoma patients. ( C ) Extracellular HSPs, PD-1, and CTLA-4 in indolent and aggressive lymphoma patients. The fold change in MFI was calculated by normalizing the activated to the resting T cells. ( D ) STAT3 expression in resting and activated T cells in healthy controls and lymphoma patients. Data represents mean ± SEM. ns, not significant, * p < 0.05, ** p < 0.01.
Article Snippet: T cells were also stained with anti-human CD279/PD-1 (REA1165, Miltenyi Biotec, Germany) (PE), CD152/CTLA-4 (REA1003, Miltenyi Biotec, Germany) (PE),
Techniques: Expressing, Fluorescence, Isolation